Review



tspan11  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Addgene inc tspan11
    ( A ) Schematic of biolayer interferometry (BLI) setup for <t>Tspan12-Norrin</t> binding: Tspan12 lacking the C-terminal tail (∆C), inserted into biotinylated nanodiscs, is immobilized on a streptavidin-coated biosensor, and Norrin association and dissociation are monitored in real time. ( B ) BLI traces of Norrin at indicated concentrations binding to and dissociating from Tspan12. ( C ) Steady-state binding curve fit to Norrin-Tspan12 binding (mean ± SD from three independent replicates at each concentration of Norrin) gives a K D of 10.4±1.2 nM (mean ± SEM). ( D ) Observed association rate constant (K obs ), determined from fitting BLI association traces (mean ± SD in three independent experiments), is linearly dependent on Norrin concentration with a slope K on = 0.00019 ± 0.00003 nM –1 s –1 (mean ± SEM). When combined with the K off = 0.0014 ± 0.00016 s –1 (mean ± SEM) determined from fitting the dissociation traces, we obtain a kinetic K D of 7.4±1.4 nM (mean ± SEM). ( E ) BLI traces of the soluble MBP-tagged Tspan12 LEL domain, at the indicated concentrations, associating to and dissociating from a biosensor loaded with MBP-tagged Norrin. Kinetic fitting gives an apparent affinity of 16±3 nM (mean ± SEM). ( F ) BLI traces of 10, 32, or 100 nM Norrin show no binding to a biosensor loaded with a nanodisc-embedded chimeric Tspan12 with the LEL replaced by that of <t>Tspan11.</t> Figure 1—source data 1. Steady-state interference shift and K obs values used to generate .
    Tspan11, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tspan11/product/Addgene inc
    Average 93 stars, based on 2 article reviews
    tspan11 - by Bioz Stars, 2026-05
    93/100 stars

    Images

    1) Product Images from "The co-receptor Tetraspanin12 directly captures Norrin to promote ligand-specific β-catenin signaling"

    Article Title: The co-receptor Tetraspanin12 directly captures Norrin to promote ligand-specific β-catenin signaling

    Journal: eLife

    doi: 10.7554/eLife.96743

    ( A ) Schematic of biolayer interferometry (BLI) setup for Tspan12-Norrin binding: Tspan12 lacking the C-terminal tail (∆C), inserted into biotinylated nanodiscs, is immobilized on a streptavidin-coated biosensor, and Norrin association and dissociation are monitored in real time. ( B ) BLI traces of Norrin at indicated concentrations binding to and dissociating from Tspan12. ( C ) Steady-state binding curve fit to Norrin-Tspan12 binding (mean ± SD from three independent replicates at each concentration of Norrin) gives a K D of 10.4±1.2 nM (mean ± SEM). ( D ) Observed association rate constant (K obs ), determined from fitting BLI association traces (mean ± SD in three independent experiments), is linearly dependent on Norrin concentration with a slope K on = 0.00019 ± 0.00003 nM –1 s –1 (mean ± SEM). When combined with the K off = 0.0014 ± 0.00016 s –1 (mean ± SEM) determined from fitting the dissociation traces, we obtain a kinetic K D of 7.4±1.4 nM (mean ± SEM). ( E ) BLI traces of the soluble MBP-tagged Tspan12 LEL domain, at the indicated concentrations, associating to and dissociating from a biosensor loaded with MBP-tagged Norrin. Kinetic fitting gives an apparent affinity of 16±3 nM (mean ± SEM). ( F ) BLI traces of 10, 32, or 100 nM Norrin show no binding to a biosensor loaded with a nanodisc-embedded chimeric Tspan12 with the LEL replaced by that of Tspan11. Figure 1—source data 1. Steady-state interference shift and K obs values used to generate .
    Figure Legend Snippet: ( A ) Schematic of biolayer interferometry (BLI) setup for Tspan12-Norrin binding: Tspan12 lacking the C-terminal tail (∆C), inserted into biotinylated nanodiscs, is immobilized on a streptavidin-coated biosensor, and Norrin association and dissociation are monitored in real time. ( B ) BLI traces of Norrin at indicated concentrations binding to and dissociating from Tspan12. ( C ) Steady-state binding curve fit to Norrin-Tspan12 binding (mean ± SD from three independent replicates at each concentration of Norrin) gives a K D of 10.4±1.2 nM (mean ± SEM). ( D ) Observed association rate constant (K obs ), determined from fitting BLI association traces (mean ± SD in three independent experiments), is linearly dependent on Norrin concentration with a slope K on = 0.00019 ± 0.00003 nM –1 s –1 (mean ± SEM). When combined with the K off = 0.0014 ± 0.00016 s –1 (mean ± SEM) determined from fitting the dissociation traces, we obtain a kinetic K D of 7.4±1.4 nM (mean ± SEM). ( E ) BLI traces of the soluble MBP-tagged Tspan12 LEL domain, at the indicated concentrations, associating to and dissociating from a biosensor loaded with MBP-tagged Norrin. Kinetic fitting gives an apparent affinity of 16±3 nM (mean ± SEM). ( F ) BLI traces of 10, 32, or 100 nM Norrin show no binding to a biosensor loaded with a nanodisc-embedded chimeric Tspan12 with the LEL replaced by that of Tspan11. Figure 1—source data 1. Steady-state interference shift and K obs values used to generate .

    Techniques Used: Binding Assay, Concentration Assay



    Similar Products

    tspan11  (Addgene inc)
    93
    Addgene inc tspan11
    ( A ) Schematic of biolayer interferometry (BLI) setup for <t>Tspan12-Norrin</t> binding: Tspan12 lacking the C-terminal tail (∆C), inserted into biotinylated nanodiscs, is immobilized on a streptavidin-coated biosensor, and Norrin association and dissociation are monitored in real time. ( B ) BLI traces of Norrin at indicated concentrations binding to and dissociating from Tspan12. ( C ) Steady-state binding curve fit to Norrin-Tspan12 binding (mean ± SD from three independent replicates at each concentration of Norrin) gives a K D of 10.4±1.2 nM (mean ± SEM). ( D ) Observed association rate constant (K obs ), determined from fitting BLI association traces (mean ± SD in three independent experiments), is linearly dependent on Norrin concentration with a slope K on = 0.00019 ± 0.00003 nM –1 s –1 (mean ± SEM). When combined with the K off = 0.0014 ± 0.00016 s –1 (mean ± SEM) determined from fitting the dissociation traces, we obtain a kinetic K D of 7.4±1.4 nM (mean ± SEM). ( E ) BLI traces of the soluble MBP-tagged Tspan12 LEL domain, at the indicated concentrations, associating to and dissociating from a biosensor loaded with MBP-tagged Norrin. Kinetic fitting gives an apparent affinity of 16±3 nM (mean ± SEM). ( F ) BLI traces of 10, 32, or 100 nM Norrin show no binding to a biosensor loaded with a nanodisc-embedded chimeric Tspan12 with the LEL replaced by that of <t>Tspan11.</t> Figure 1—source data 1. Steady-state interference shift and K obs values used to generate .
    Tspan11, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tspan11/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    tspan11 - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier
    rnascope probe: tspan11-rn [c4]  (Advanced Cell Diagnostics Inc)
    90
    Advanced Cell Diagnostics Inc rnascope probe: tspan11-rn [c4]
    ( A ) Schematic of biolayer interferometry (BLI) setup for <t>Tspan12-Norrin</t> binding: Tspan12 lacking the C-terminal tail (∆C), inserted into biotinylated nanodiscs, is immobilized on a streptavidin-coated biosensor, and Norrin association and dissociation are monitored in real time. ( B ) BLI traces of Norrin at indicated concentrations binding to and dissociating from Tspan12. ( C ) Steady-state binding curve fit to Norrin-Tspan12 binding (mean ± SD from three independent replicates at each concentration of Norrin) gives a K D of 10.4±1.2 nM (mean ± SEM). ( D ) Observed association rate constant (K obs ), determined from fitting BLI association traces (mean ± SD in three independent experiments), is linearly dependent on Norrin concentration with a slope K on = 0.00019 ± 0.00003 nM –1 s –1 (mean ± SEM). When combined with the K off = 0.0014 ± 0.00016 s –1 (mean ± SEM) determined from fitting the dissociation traces, we obtain a kinetic K D of 7.4±1.4 nM (mean ± SEM). ( E ) BLI traces of the soluble MBP-tagged Tspan12 LEL domain, at the indicated concentrations, associating to and dissociating from a biosensor loaded with MBP-tagged Norrin. Kinetic fitting gives an apparent affinity of 16±3 nM (mean ± SEM). ( F ) BLI traces of 10, 32, or 100 nM Norrin show no binding to a biosensor loaded with a nanodisc-embedded chimeric Tspan12 with the LEL replaced by that of <t>Tspan11.</t> Figure 1—source data 1. Steady-state interference shift and K obs values used to generate .
    Rnascope Probe: Tspan11 Rn [C4], supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnascope probe: tspan11-rn [c4]/product/Advanced Cell Diagnostics Inc
    Average 90 stars, based on 1 article reviews
    rnascope probe: tspan11-rn [c4] - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier
    tspan11  (Proteintech)
    92
    Proteintech tspan11
    Expression box diagram of gene expression in pan-cancer. a – f The gene expression of HOXD9, CPRC5B, ITLN1, CXCL13, <t>TSPAN11</t> and TIMP1 in different tumors, respectively
    Tspan11, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tspan11/product/Proteintech
    Average 92 stars, based on 1 article reviews
    tspan11 - by Bioz Stars, 2026-05
    92/100 stars
    		  Buy from Supplier
    unique arrangement of bone matrix orthogonal to osteoblast alignment controlled by tspan11-mediated focal adhesion assembly  (Canon inc)
    90
    Canon inc unique arrangement of bone matrix orthogonal to osteoblast alignment controlled by tspan11-mediated focal adhesion assembly
    Expression box diagram of gene expression in pan-cancer. a – f The gene expression of HOXD9, CPRC5B, ITLN1, CXCL13, <t>TSPAN11</t> and TIMP1 in different tumors, respectively
    Unique Arrangement Of Bone Matrix Orthogonal To Osteoblast Alignment Controlled By Tspan11 Mediated Focal Adhesion Assembly, supplied by Canon inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/unique arrangement of bone matrix orthogonal to osteoblast alignment controlled by tspan11-mediated focal adhesion assembly/product/Canon inc
    Average 90 stars, based on 1 article reviews
    unique arrangement of bone matrix orthogonal to osteoblast alignment controlled by tspan11-mediated focal adhesion assembly - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier
    flag-tspan11  (OriGene)
    90
    OriGene flag-tspan11
    Expression box diagram of gene expression in pan-cancer. a – f The gene expression of HOXD9, CPRC5B, ITLN1, CXCL13, <t>TSPAN11</t> and TIMP1 in different tumors, respectively
    Flag Tspan11, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flag-tspan11/product/OriGene
    Average 90 stars, based on 1 article reviews
    flag-tspan11 - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier
    flag tspan11  (OriGene)
    90
    OriGene flag tspan11
    Expression box diagram of gene expression in pan-cancer. a – f The gene expression of HOXD9, CPRC5B, ITLN1, CXCL13, <t>TSPAN11</t> and TIMP1 in different tumors, respectively
    Flag Tspan11, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flag tspan11/product/OriGene
    Average 90 stars, based on 1 article reviews
    flag tspan11 - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Schematic of biolayer interferometry (BLI) setup for Tspan12-Norrin binding: Tspan12 lacking the C-terminal tail (∆C), inserted into biotinylated nanodiscs, is immobilized on a streptavidin-coated biosensor, and Norrin association and dissociation are monitored in real time. ( B ) BLI traces of Norrin at indicated concentrations binding to and dissociating from Tspan12. ( C ) Steady-state binding curve fit to Norrin-Tspan12 binding (mean ± SD from three independent replicates at each concentration of Norrin) gives a K D of 10.4±1.2 nM (mean ± SEM). ( D ) Observed association rate constant (K obs ), determined from fitting BLI association traces (mean ± SD in three independent experiments), is linearly dependent on Norrin concentration with a slope K on = 0.00019 ± 0.00003 nM –1 s –1 (mean ± SEM). When combined with the K off = 0.0014 ± 0.00016 s –1 (mean ± SEM) determined from fitting the dissociation traces, we obtain a kinetic K D of 7.4±1.4 nM (mean ± SEM). ( E ) BLI traces of the soluble MBP-tagged Tspan12 LEL domain, at the indicated concentrations, associating to and dissociating from a biosensor loaded with MBP-tagged Norrin. Kinetic fitting gives an apparent affinity of 16±3 nM (mean ± SEM). ( F ) BLI traces of 10, 32, or 100 nM Norrin show no binding to a biosensor loaded with a nanodisc-embedded chimeric Tspan12 with the LEL replaced by that of Tspan11. Figure 1—source data 1. Steady-state interference shift and K obs values used to generate .

    Journal: eLife

    Article Title: The co-receptor Tetraspanin12 directly captures Norrin to promote ligand-specific β-catenin signaling

    doi: 10.7554/eLife.96743

    Figure Lengend Snippet: ( A ) Schematic of biolayer interferometry (BLI) setup for Tspan12-Norrin binding: Tspan12 lacking the C-terminal tail (∆C), inserted into biotinylated nanodiscs, is immobilized on a streptavidin-coated biosensor, and Norrin association and dissociation are monitored in real time. ( B ) BLI traces of Norrin at indicated concentrations binding to and dissociating from Tspan12. ( C ) Steady-state binding curve fit to Norrin-Tspan12 binding (mean ± SD from three independent replicates at each concentration of Norrin) gives a K D of 10.4±1.2 nM (mean ± SEM). ( D ) Observed association rate constant (K obs ), determined from fitting BLI association traces (mean ± SD in three independent experiments), is linearly dependent on Norrin concentration with a slope K on = 0.00019 ± 0.00003 nM –1 s –1 (mean ± SEM). When combined with the K off = 0.0014 ± 0.00016 s –1 (mean ± SEM) determined from fitting the dissociation traces, we obtain a kinetic K D of 7.4±1.4 nM (mean ± SEM). ( E ) BLI traces of the soluble MBP-tagged Tspan12 LEL domain, at the indicated concentrations, associating to and dissociating from a biosensor loaded with MBP-tagged Norrin. Kinetic fitting gives an apparent affinity of 16±3 nM (mean ± SEM). ( F ) BLI traces of 10, 32, or 100 nM Norrin show no binding to a biosensor loaded with a nanodisc-embedded chimeric Tspan12 with the LEL replaced by that of Tspan11. Figure 1—source data 1. Steady-state interference shift and K obs values used to generate .

    Article Snippet: Human Tspan12 (DNASU) truncated after the fourth transmembrane domain (∆C, residues 1–252) and Tspan12 with the LEL replaced by that of TSPAN11 (Tspan12-LEL11, Addgene plasmid #115785 from Harald Junge; ) were C-terminally tagged with the Rho-1D4 antibody recognition sequence TETSQVAPA.

    Techniques: Binding Assay, Concentration Assay

    Expression box diagram of gene expression in pan-cancer. a – f The gene expression of HOXD9, CPRC5B, ITLN1, CXCL13, TSPAN11 and TIMP1 in different tumors, respectively

    Journal: Cancer Cell International

    Article Title: Identification and development of a novel invasion-related gene signature for prognosis prediction in colon adenocarcinoma

    doi: 10.1186/s12935-021-01795-1

    Figure Lengend Snippet: Expression box diagram of gene expression in pan-cancer. a – f The gene expression of HOXD9, CPRC5B, ITLN1, CXCL13, TSPAN11 and TIMP1 in different tumors, respectively

    Article Snippet: We used primary antibodies raised against GAPDH, CXCL13, ITLN1, HOXD9, and GPRC5B (Santa-Cruz Biotechnology, CA, USA), as well as TIMP1 and TSPAN11 (Proteintech, China).

    Techniques: Expressing, Gene Expression